TransformationofAgrobacteriumThawthecompetentAgrobacteriumoniceandadd2gDNA,mix(notvertex)Incubate5minoniceFreezeinliquidnitrogenfor5minIncubateat37oCfor5minAddedthe1mlprewarmed(28oC)YEPmediumShakefor2–4hoursat28oCPlate5x200µlaliquitsonYEPPlate(withrifandkan)Incubateplateat28oCforca2days.(normallyyouwillget250cfu/µgifplasmidqualityisgood)--------YEPplate500mlYEPmedium+500µlRif(50mg/ml)+500µlkann(50µmg{ml)
