实验十一Western杂交(WesternBlot)一、【实验目的】了解蛋白质印迹的方法和操作要点。二、【实验原理】蛋白质印迹法一般是将SDS-聚丙烯酰胺凝胶电泳分离的蛋白质分子区带通过一定的方法转移并固定到一种特殊的载体上,使之成为稳定的、经得起各种处理并容易检出的固定化生物大分子,常用的转移方法有电转移、毛细管转移等,常用载体有硝酸纤维素膜(NC膜)、PVDF膜、尼龙膜等,它们与生物大分子都是共价结合。然后通过特异性...
Section_ⅣOther_Parts_of_the_Module[原文呈现][读文清障]Peoplegenerally①agreethatPabloPicasso,wholivedfrom1881to1973,isthetwentiethcentury’sgreatestWesternartist.HewasborninSpain②andattheageof③tenwasalreadyanexcellent④①generallyadv.一般地,普遍地②Spainn.西班牙③attheageof在岁时④excellentadj.极好的,秀的1[原文呈现][读文清障]⑤exhibition/ˌeksIbIʃn/n.展览⑥moveto搬到⑦inone’searlytwenti...
Myparentsarefondofgoingtoartgalleriestowatchtheexhibitions.Theyoftentakemewiththem,whichisaimedatdevelopingmylikeforpainting.Ioncestudieddrawinglandscapeandwatercolor,butsoonIwastiredofthemandfinallygavethemup.However,Istillenjoywatchingfamousartists’works.Icantellwhoseworkstheyarebythestyles.1MyfavouriteartistsarePabloPicassoandQiBaishi.Theytwohaddifferentstyles.PabloPicassowonunusualsucce...
Unit3Languageinuse11.Metoo.(教材P77)我也是。[解读]Metoo用于口语中,表示与前面提到的情况一样,意为:我也是;我也如此。相当于I...,too.或so引导的倒装句。—Ilikepopmusic.我喜欢流行音乐。—Metoo./Ilikeit,too./SodoI.我也是。拓展如果是针对否定的情况,表示“我也不”,要用Meneither.表达。—Iamnotinterestedinclassicalmusic.我对古典音乐不感兴趣。—Meneither.我也不感兴趣。2名师解题342.Everyyear,theViennaNewYear...
Module12WesternmusicUnit3Languageinuse1Whatabeautifulcity!DoyouliketraditionalWesternmusicorpopmusic?Revision2Whatabigpencilboxitis!3Whataprettygirlsheis!4Whatbeautifulflowerstheyare!5Whatlovelypandas(theyare)!Wow,whatabigpumpkin!6Thecatislovely.Whatalovelycatitis!7—Istheyellowrulershortorlong?—It’s_________.long8—Cansheskatewellorbadly?—Shecanskate______.well9—Doyougotoschoolbybikeo...
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Unit1It’ssobeautiful!Module12Westernmusic1Let’slearnsomeinstruments.guitar/gi’ta:/violin/vai’lin/ə2pianodrum/drΛm/3ErhuChinesezither古筝4•Doyoulikemusic?•Whatkindsofmusicdoyouknow?•Whichkindofmusicdoyoulikebest?5RBpoprockRapHip-hopJazzPunkcountrymusicBluesfolkmusicclassicalmusicmusic6Whatkindofmusicisthis?Beijingopera7rockWhatkindofmusicisthis?8traditionalWesternmusicBeethoven9popmusic...
Unit2ViennaisthecentreofEuropeanclassicalmusic.Module12Westernmusic1ViennaisthecentreofEuropeanclassicalmusic.2ViennaisabeautifuloldcityontheRiverDanube.3ItsthecapitalcityofAustriaandthecenterofEuropeanclassicalmusic.4MusicAppreciation5MozartTheMarriageofFigaro《费加罗的婚礼》6矗立在维也纳皇家公园的莫扎特雕像7JohannStraussTheBlueDanube《蓝色的多瑙河》VoicesofSpring,Op.《春之声》圆舞曲8城市公园...
Section_ⅤWriting—_主题句主题句一般位于段首,也可置于段末或段中。主题句为一个完整的句子,用以概括、叙述和说明该段的主题。它通常是一个观点(opinion),而不是一个事实(fact)。主题句不仅会让文章条理分明,还能够很好地诠释文章主题思想,可谓是文章的灵魂。所以,写好主题句对写好一篇文章有着至关重要的作用。1一、注意事项1.主题句要概括一定内容,可以用扩展句来说明和支持它;2.尽量使用简单句或简洁明了的句子;...
Unit2ViennaisthecentreofEuropeanclassicalmusic.11.HisdancemusicmadehimfamousalloverEurope.(教材P74)他的舞曲使他闻名于整个欧洲。[解读]本句是含有宾语补足语的复合句,其句子结构如下图所示:Hisdancemusicmadehim{famousalloverEurope}.↑↑↑↑主语谓语宾语宾语补足语宾语补足语是对宾语的解释说明。2拓展make表“使役动词”的用法小结(1)make+sb/sth+adj.意为“使某人/某物”。(2)make+sb/sth+n.意为“使某人/某物成为...
七年级英语下册 Module 12 Western music检测卷课件 (新版)外研版[共23页]
Module12Westernmusic1Unit1It’ssobeautiful!21.Hmm,thisisWesternmusic,isn’tit?(教材P72)嗯,这是西方音乐,不是吗?[解读]本句型前面是肯定形式,后面是否定形式,且后面句子为简短的小问句。这种问句叫反意疑问句。反意疑问句是由陈述句加上简短的疑问句构成。反意疑问句遵循“前肯后否,前否后肯”的原则。Hedoesn’tlikemusic,doeshe?他不喜欢音乐,是吗?MikeisEnglish,isn’the?迈克是英国人,不是吗?3名师解题452.Well,Ilikeb...
WesternBlot详解(原理、分类、试剂、步骤及问题解答)Western免疫印迹〔WesternBlot〕是将蛋白质转移到膜上,然后利用抗体进展检测。对表达蛋白,可用相应抗体作为一抗进展检测,对基因的表达产物,可通过融合局部的抗体检测。本文主要通过以下几个方面来具体地介绍一下WesternBlot技术:一、原理二、分类i.放射自显影ii.底物化学发光ECLECFiv.底物DAB呈色三、主要试剂四、主要步骤五、试验常见的问题指南1.参考书推举2.针对样品...